Actor Michael Muhney was initially up for the role of JD, but was told he wasn't quirky enough for the role.

Sacred Heart Hospital is named after the school which Christa Miller, (wife of writer Bill Lawrence) attended.

The teasers for the 2001 season finale parodied the teasers for the show "24" (2001) which had the same time slot as "Scrubs" (2001).

As an experiment, the Season 5 finale "My Transition" (#5.24) was finished and aired in High Definition. "Scrubs" (2001) is the first network show to complete an on-line edit in Final Cut Pro SD, and with this episode becomes the first network show to on-line in HD in Final Cut Pro. However the show continues to be produced in Standard Definition. According to the producer, Randall Winston: "After a lot of teasing, yes, the HD was an experiment, for everyone really. We will not be doing any more HD - there is a cost that no one is interested in covering and there is debate, not among us here at the show, about how the quality stands up. I don't think that we can get the look that the show is designed around [by using] tape."

The network leased and refurbished a closed hospital (North Hollywood Medical Center in the San Fernando Valley in California) for the program. The lower and upper floors of the hospital are used as other sets and production offices.

At the beginning of the second season, a longer intro was introduced that included all of the characters in the show, and not just the doctors. It was quickly scuttled when NBC decided to extend the length of the episodes in an attempt to win more viewers.

In the Spanish dubbed version of the show, the nurse Carla Espinosa speaks with her mother in Italian. In the original English version, they speak Spanish.

Ted's "Band" is in fact, "The Blanks" a group the actor formed with his friends at college.

"Scrubs" (2001) was originally going to air on ABC but then ABC turned it down and NBC picked up the show. In 2008, however, ABC picked it up after NBC canceled it.

Most of the lead cast (and many of the recurring characters) are also supporting/ lead or guest voices on "Clone High U.S.A." (2002). Both are executive produced by Bill Lawrence.

Former members of "Spin City" (1996) often appear as guest stars. Cast members have included Michael J. Fox, Heather Locklear, Barry Bostwick, Richard Kind, Alan Ruck, Alexander Chaplin, and Michael Boatman. "Scrubs" (2001) creator Bill Lawrence was also the creator of Spin City, and producer Randall Winston was also an associate producer for Spin City.

For the first eight seasons almost every episode title begins with the word "My". Notable exceptions are the episodes entitled "His Story", "His Story II", "Her Story", "Her Story II", "His Story III" and ''His Story IV'' These episodes each contained internal narration from a character other than JD. (Dr. Cox, Turk, Elliot, Carla, The Janitor and Dr. Kelso respectively). There is also "Their Story" in which we hear the thoughts of Ted, the Todd and Jordan. In "Their Story II" the thoughts of 3 interns are followed. Starting with season nine every episode title starts with "Our".

Dr. Cox's full name is Percival Ulysses, but he goes by Perry.

The show has some connections to the abbreviation TCW. Donald Faison starred in Remember The Titans whose high school was TCW. JD later dates a girl whose is called TCW (Tasty Coma Wife) and then it is shown in the episode My No Good Reason as being from a school with the initials TCW.

Dr. Cox's son, Jack, was born March 21, 2003.

All the plaques on that wall are for the pets of the cast & crew of "Scrubs" (2001).

Through the first season, the cast and crew, especially writer/creator Bill Lawrence, were so sure that the series would be canceled by the end of the season, that a plot twist was created for use when they'd have a forced series finale. This plot twist noted the fact that the Janitor, Neil Flynn's character, never directly spoke nor was spoken to by any other character than JD, Zach Braff's character. Therefore, the Janitor would be a complete figment of JD's imagination. This idea was kept up into the second season, still in fear of cancellation, until Flynn asked Lawrence to be able to finally interact with another actor on the series. (Although Elliot actually throws a cup at him in the 6th episode and sarcastically says "SORRY!", in episode 11 he gets punched on the balls by a little girl and in episode 15 the janitor teases the nurse with the vacuum cleaner)

Bill Lawrence and a few of the writers jokingly claim that Neil Flynn (The Janitor) has never actually spoken a line from any script. A fourth season script reportedly even contained "(Whatever Neil says)". While Lawrence's comment is certainly an exaggeration, it illustrates how much of his performance is spontaneous.

JD's father's (John Ritter) final line when he asks JD to pull his finger and then says, "I pooed a little," was totally improvised and Zach Braff had to bite the insides of his cheeks to stop himself laughing.