Mickey Mouse in 1928 appearing in the short, 'The Gallopin' Gaucho'. According to Wikipedia, it was the second film featuring Mickey Mouse to be produced, following Plane Crazy (released 2nd) and preceding Steamboat Willie (released 1st). The Walt Disney Company completed the silent version on Aug. 7, 1928, but failed to distribute it widely. Due to a lack of interest, the film was not given wide release until after 'Steamboat Willie' and 'Plane Crazy' had been released, making it the third Mickey Mouse short, with the sound version released on Dec. 30, 1928. You can view the animated short from YouTube below.
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